+ SFB860 Integrative Structural Biology of Dynamic Macromolecular Assemblies
sfb860Goettingen UniversityMax-Plank-Institute

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Press releases> Press release



Prof. Dr. Ralf Ficner mail
Tel.: +49 (0)551-39-14072
Fax: +49 (0)551-39-14082

SFB860 Administration
Susanne van Beckum mail
Tel.: +49 (0)551-39-10972
Fax: +49 (0)551-39-14082

Abtl. Molekulare Strukturbiologie
Georg-August-Universität Göttingen
Justus-von-Liebig-Weg 11
37077 Göttingen

Introduction of SFB860

The dynamics of large macromolecular assemblies is a fundamental property that has great impact on their molecular and cellular function. This CRC will analyze the dynamics of several large macromolecular complexes with regard to their structures, their changes in conformation and composition, their interactions with other biological macromolecules, and with respect to their temporal and spatial location in the cell. Hence, the projects of the CRC focus on the determination of three-dimensional structures, the deduction of structure’s implication for the molecular and cellular function, the dynamic processes during assembly, remodelling and disassembly, the role of natively unfolded domains, the impact of posttranslational modifications on structure, function and dynamics, the conformational dynamics of functional complexes and their subunits, and kinetics and thermodynamics of macromolecular interactions. Studying the three-dimensional structure and the dynamics of single, average-size proteins is nowadays mostly a routine. However, due to the stunning complexity of large multi-protein- and ribonucleoprotein complexes, classical biochemical, biophysical and structure determination methods - when used exclusively - do not meet the requirements to comprehend such systems. Innovative strategies have to be developed for sample preparation, identification of macromolecular interaction networks, as well as for the analysis of compositional and structural dynamics. Hybrid approaches that combine single particle electron microscopy, crystallography, NMR spectroscopy, small angle X-ray scattering, single-molecule techniques and computational tools are required to generate testable atomic models of large macromolecular complexes. The CRC will bundle a wide spectrum of interdisciplinary competences present in Göttingen for the integrative analysis of a defined set of large macromolecular complexes, namely the spliceosome, the ribosome, the RNA degradosome, the mRNP locasome, the slam RNP, the mitochondrial translocase complex, nuclear export complexes, autophagy protein complexes, SLP-assembled signalosomes, the COP9 signalosome and the pyruvate dehydrogenase mulitenzyme complex. Since challenges and approaches are very similar for these functionally different macro¬molecular assemblies, the application of complementary biophysical and biochemical methods, as well as the exchange of experimental experiences and know-how will result in a significant added value for each project.

SFB 860 is funded by DFG (Deutsche Forschungsgemeinschaft)
1. funding period July 2010 - June 2014.
2. funding period July 2014 - June 2018.


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Publications> all Publications

  • in press
    Sem1 links proteasome stability and specificity to multicellular development.
  • 12.2017
    Three-Dimensional Reconstruction of Nuclear Envelope Architecture Using Dual-Color Metal-Induced Energy Transfer Imaging.
  • 12.2017
    Capturing the Asc1p/RACK1 microenvironment at the head region of the 40S ribosome with quantitative BioID.
  • 12.2017
    Slam protein dictates subcellular localization and translation of its own RNA.
  • 11.2017
    Mechanism of Transcription Anti-termination in Human Mitochondria.
  • 11.2017
    Structural Basis of Mitochondrial Transcription Initiation.
  • 11.2017
    Structures of transcription pre-initiation complex with TFIIH and Mediator.
  • 11.2017
    INA complex liaises the F1Fo-ATP synthase membrane motor modules.

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