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2021

RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation.

Aquino GR, Krogh N, Hackert P, Martin R, Davila Gallesio J, van Nues RW, Schneider C, Watkins NJ, Nielsen H, Bohnsack KE, Bohnsack MT.
Nucleic Acids Res. 2021 Apr 19;49(7):4066-4084. doi: 10.1093/nar/gkab159.

The RNA-binding protein FUS is chaperoned and imported into the nucleus by a network of import receptors.

Baade I, Hutten H, Sternburg EL, Pörschke M, Hofweber M, Dormann D, Kehlenbach RH.
J Biol Chem. 2021 Apr 12;100659. doi: 10.1016/j.jbc.2021.100659. Online ahead of print.

The structure of Prp2 bound to RNA and ADP-BeF 3- reveals structural features important for RNA unwinding by DEAH-box ATPases.

Hamann F, Zimmerningkat LC, Becker RA, Garbers TB, Neumann P, Hub JS, Ficner R.
Acta Crystallogr D Struct Biol. 2021 Apr 1;77(Pt 4):496-509. doi: 10.1107/S2059798321001194. Epub 2021 Mar 30.

A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex.

Hamed M, Caspar B, Port SA, Kehlenbach RH.
J Cell Sci. 2021 Mar 26;134(6):jcs258095. doi: 10.1242/jcs.258095.

Crystal structure of human CRM1, covalently modified by 2-mercaptoethanol on Cys528, in complex with RanGTP.

Shaikhqasem A, Schmitt K, Valerius O, Ficner R.
Acta Crystallogr F Struct Biol Commun. 2021 Mar 1;77(Pt 3):70-78. doi: 10.1107/S2053230X2100203X. Epub 2021 Mar 3.

Ion type and valency differentially drive vimentin tetramers into intermediate filaments or higher order assemblies.

Denz M, Marschall M, Herrmann H, Köster S.
Soft Matter. 2021 Jan 28;17(4):870-878. doi: 10.1039/d0sm01659d. Epub 2020 Nov 25.

Disease-Associated Tau Phosphorylation Hinders Tubulin Assembly within Tau Condensates.

Savastano A, Flores D, Kadavath H, Biernat J, Mandelkow E, Zweckstetter M.
Angew Chem Int Ed Engl. 2021 Jan 11;60(2):726-730. doi: 10.1002/anie.202011157. Epub 2020 Nov 9.

Nuclear SR-protein mediated mRNA quality control is continued in cytoplasmic nonsense-mediated decay.

Grosse S, Lu Y-Y, Coban I, Neumann B, Krebber H.
RNA Biol. 2021 Jan 7;1-18. doi: 10.1080/15476286.2020.1851506. Online ahead of print

Post-translational modifications soften vimentin intermediate filaments.

Kraxner J, Lorenz C, Menzel J, Parfentev I, Silbern I, Denz M, Urlaub H, Schwappach B, Köster S.
Nanoscale. 2021 Jan 7;13(1):380-387. doi: 10.1039/d0nr07322a. Epub 2020 Dec 22.

Regulation of DEAH-box RNA helicases by G-patch proteins.

Bohnsack KE, Ficner R, Bohnsack MT, Jonas S.
Biol Chem. 2021 Jan 6;402(5):561-579. doi: 10.1515/hsz-2020-0338. Print 2021 Apr 27.

2020

Molecular basis of the interaction of Hsp90 with its co-chaperone Hop.

Lott A, Oroz J, Zweckstetter M.
Protein Sci. 2020 Dec;29(12):2422-2432. doi: 10.1002/pro.3969. Epub 2020 Oct 19.

Dual function of GTPBP6 in biogenesis and recycling of human mitochondrial ribosomes.

Lavdovskaia E, Denks K, Nadler F, Steube E, Linden A, Urlaub H, Rodnina MV, Richter-Dennerlein R.
Nucleic Acids Res. 2020 Dec 16;48(22):12929-12942. doi: 10.1093/nar/gkaa1132.

Atomic-resolution protein structure determination by cryo-EM.

Yip KM, Fischer N, Paknia E, Chari A, Stark H.
Nature. 2020 Nov;587(7832):157-161. doi: 10.1038/s41586-020-2833-4. Epub 2020 Oct 21.

Hülle Cells of Aspergillus nidulans with Nuclear Storage and Developmental Backup Functions Are Reminiscent of Multipotent Stem Cells.

Troppens DM, Köhler AM, Schlüter R, Hoppert M, Gerke J, Braus GH.
mBio. 2020 Aug 11;11(4):e01673-20. doi: 10.1128/mBio.01673-20.

Crystal structure of the Rab33B/Atg16L1 effector complex.

Metje-Sprink J, Groffmann J, Neumann P, Barg-Kues B, Ficner R, Kühnel K, Schalk AM, Binotti B.
Sci Rep. 2020 Jul 31;10(1):12956. doi: 10.1038/s41598-020-69637-0.

Characterization of Inhibition Reveals Distinctive Properties for Human and Saccharomyces cerevisiae CRM1.

Shaikhqasem A, Dickmanns A, Neumann P, Ficner R.
J Med Chem. 2020 Jul 23;63(14):7545-7558. doi: 10.1021/acs.jmedchem.0c00143. Epub 2020 Jul 7.

Molecular architecture of the human 17S U2 snRNP.

Zhang Z, Will CL, Bertram K, Dybkov O, Hartmuth K, Agafonov DE, Hofele R, Urlaub H, Kastner B, Lührmann R, Stark H.
Nature. 2020 Jul;583(7815):310-313. doi: 10.1038/s41586-020-2344-3. Epub 2020 Jun 3.

Atg21 organizes Atg8 lipidation at the contact of the vacuole with the phagophore.

Munzel L, Neumann P, Otto FB, Krick R, Metje-Sprink J, Kroppen B, Karedla N, Enderlein J, Meinecke M, Ficner R, Thumm M.
Autophagy. 2020 Jun 9;1-21. doi: 10.1080/15548627.2020.1766332. Online ahead of print.

A cross-linking mass spectrometry approach defines protein interactions in yeast mitochondria.

Linden A, Deckers M, Parfentev I, Pflanz R, Homberg B, Neumann P, Ficner R, Rehling P, Urlaub H.
Mol Cell Proteomics. 2020 Apr 24. pii: mcp.RA120.002028. doi: 10.1074/mcp.RA120.002028.

Bayesian inference of chromatin structure ensembles from population-averaged contact data.

Carstens S, Nilges M, Habeck M.
Proc Natl Acad Sci U S A. 2020 Apr 7;117(14):7824-7830. doi: 10.1073/pnas.1910364117. Epub 2020 Mar 19.

Determination of Complex Small-Molecule Structures Using Molecular Alignment Simulation.

Ibáñez de Opakua A, Klama F, Ndukwe IE, Martin GE, Williamson RT, Zweckstetter M.
Angew Chem Int Ed Engl. 2020 Apr 6;59(15):6172-6176. doi: 10.1002/anie.202000311. Epub 2020 Feb 24.

The Molecular Basis of the Interaction of Cyclophilin A with α-Synuclein.

Favretto F, Baker JD, Strohäker T, Andreas LB, Blair LJ, Becker S, Zweckstetter M.
Angew Chem Int Ed Engl. 2020 Mar 27;59(14):5643-5646. doi: 10.1002/anie.201914878. Epub 2020 Jan 29.

Discovery of a Regulatory Subunit of the Yeast Fatty Acid Synthase.

Singh K, Graf B, Linden A, Sautner V, Urlaub H, Tittmann K, Stark H, Chari A.
Cell. 2020 Mar 19;180(6):1130-1143.e20. doi: 10.1016/j.cell.2020.02.034. Epub 2020 Mar 10.

From TOM to the TIM23 complex – handing over of a precursor.

Callegari S, Cruz-Zaragoza LD, Rehling P.
Biol Chem. 2020 Mar 9. pii: /j/bchm.ahead-of-print/hsz-2020-0101/hsz-2020-0101.xml. doi: 10.1515/hsz-2020-0101.

Tripartite phase separation of two signal effectors with vesicles priming B cell responsiveness.

Wong LE, Bhatt A, Erdmann PS, Hou Z, Maier J, Pirkuliyeva S, Engelke M, Becker S, Plitzko J, Wienands J, Griesinger C.
Nat Commun. 2020 Feb 12;11(1):848. doi: 10.1038/s41467-020-14544-1.

Structural analysis of the intrinsically disordered splicing factor Spp2 and its binding to the DEAH-box ATPase Prp2.

Hamann F, Schmitt A, Favretto F, Hofele R, Neumann P, Xiang S, Urlaub H, Zweckstetter M, Ficner R.
Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2948-2956. doi: 10.1073/pnas.1907960117. Epub 2020 Jan 23.

Dbp5/DDX19 between Translational Readthrough and Nonsense Mediated Decay.

Beißel C, Grosse S, Krebber H.
Int J Mol Sci. 2020 Feb 6;21(3). pii: E1085. doi: 10.3390/ijms21031085.

Into the basket and beyond: the journey of mRNA through the nuclear pore complex.

Ashkenazy-Titelman A, Shav-Tal Y, Kehlenbach RH.
Biochem J. 2020 Jan 17;477(1):23-44. doi: 10.1042/BCJ20190132.

Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit.

Braun CM, Hackert P, Schmid CE, Bohnsack MT, Bohnsack KE, Perez-Fernandez J.
Nucleic Acids Res. 2020 Jan 10;48(1):405-420. doi: 10.1093/nar/gkz1079.

2019

Active role of elongation factor G in maintaining the mRNA reading frame during translation.

Peng BZ, Bock LV, Belardinelli R, Peske F, Grubmüller H, Rodnina MV.
Sci Adv. 2019 Dec 20;5(12):eaax8030. doi: 10.1126/sciadv.aax8030. eCollection 2019 Dec.

Graphene-based metal-induced energy transfer for sub-nanometre optical localization.

Ghosh A, Sharma A, Chizhik AI, Isbaner S, Ruhlandt D, Tsukanov R, Gregor I, Karedla N, Enderlein J.
Nature Photonics | VOL 13 | DECEMBER 2019 | 860–865 | www.nature.com/naturephotonics.

Thermodynamic control of -1 programmed ribosomal frameshifting.

Bock LV, Caliskan N, Korniy N, Peske F, Rodnina MV, Grubmüller H.
Nat Commun. 2019 Oct 10;10(1):4598. doi: 10.1038/s41467-019-12648-x.

The nuclear pore proteins Nup88/214 and T-cell acute lymphatic leukemia-associated NUP214 fusion proteins regulate Notch signaling.

Kindermann B, Valkova C, Krämer A, Perner B, Engelmann C, Behrendt L, Kritsch D, Jungnickel B, Kehlenbach RH, Oswald F, Englert C, Kaether C.
J Biol Chem. 2019 Aug 2;294(31):11741-11750. doi: 10.1074/jbc.RA118.006357. Epub 2019 Jun 11.

Structural and Functional Analyses of the Human PDH Complex Suggest a ‘‘Division-of-Labor’’ Mechanism by Local E1 and E3 Clusters.

Prajapati S, Haselbach D, Wittig S, Patel MS, Chari A, Schmidt C, Stark H, Tittmann K.
Structure. 2019 Jul 2;27(7):1124-1136.e4. doi: 10.1016/j.str.2019.04.009. Epub 2019 May 23.

Integration of Fungus-Specific CandA-C1 into a Trimeric CandA Complex Allowed Splitting of the Gene for the Conserved Receptor Exchange Factor of CullinA E3 Ubiquitin Ligases in Aspergilli.

Köhler AM, Harting R, Langeneckert AE, Valerius O, Gerke J, Meister C, Strohdiek A, Braus GH.
mBio. 2019 Jun 18;10(3). pii: e01094-19. doi: 10.1128/mBio.01094-19.

Nuclear Pre-snRNA Export Is an Essential Quality Assurance Mechanism for Functional Spliceosomes.

Becker D, Hirsch AG, Bender L, Lingner T, Salinas G, Krebber H.
Cell Rep. 2019 Jun 11;27(11):3199-3214.e3. doi: 10.1016/j.celrep.2019.05.031.

EF-G-induced ribosome sliding along the noncoding mRNA.

Klimova M, Senyushkina T, Samatova E, Peng BZ, Pearson M, Peske F, Rodnina MV.
Sci Adv. 2019 Jun 5;5(6):eaaw9049. doi: 10.1126/sciadv.aaw9049. eCollection 2019 Jun.

Modulation of HIV-1 Gag/Gag-Pol frameshifting by tRNA abundance.

Korniy N, Goyal A, Hoffmann M, Samatova E, Peske F, Pöhlmann S, Rodnina MV.
Nucleic Acids Res. 2019 Jun 4;47(10):5210-5222. doi: 10.1093/nar/gkz202.

Translation termination depends on the sequential ribosomal entry of eRF1 and eRF3.

Beißel C, Neumann B, Uhse S, Hampe I, Karki P, Krebber H.
Nucleic Acids Res. 2019 May 21;47(9):4798-4813. doi: 10.1093/nar/gkz177.

Structural basis for RNA translocation by DEAH-box ATPases.

Hamann F, Enders M, Ficner R.
Nucleic Acids Res. 2019 May 7;47(8):4349-4362. doi: 10.1093/nar/gkz150.

ROMO1 is a constituent of the human presequence translocase required for YME1L protease import.

Richter F, Dennerlein S, Nikolov M, Jans DC, Naumenko N, Aich A, MacVicar T, Linden A, Jakobs S, Urlaub H, Langer T, Rehling P.
J Cell Biol. 2019 Feb 4;218(2):598-614. doi: 10.1083/jcb.201806093. Epub 2018 Dec 31.

2018

RNA helicases mediate structural transitions and compositional changes in pre-ribosomal complexes.

Brüning L, Hackert P, Martin R, Davila Gallesio J, Aquino GRR, Urlaub H, Sloan KE, Bohnsack MT.
Nat Commun. 2018 Dec 19;9(1):5383. doi: 10.1038/s41467-018-07783-w.

Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence.

Bonnin E, Cabochette P, Filosa A, Jühlen R, Komatsuzaki S, Hezwani M, Dickmanns A, Martinelli V, Vermeersch M, Supply L, Martins N, Pirenne L, Ravenscroft G, Lombard M, Port S, Spillner C, Janssens S, Roets E, Van Dorpe J, Lammens M, Kehlenbach RH, Ficner R, Laing NG, Hoffmann K, Vanhollebeke B, Fahrenkrog B.
PLoS Genet. 2018 Dec 13;14(12):e1007845. doi: 10.1371/journal.pgen.1007845. eCollection 2018 Dec

Motor recruitment to the TIM23 channel’s lateral gate restricts polypeptide release into the inner membrane.

Schendzielorz AB, Bragoszewski P, Naumenko N, Gomkale R, Schulz C, Guiard B, Chacinska A, Rehling P.
Nat Commun. 2018 Oct 2;9(1):4028. doi: 10.1038/s41467-018-06492-8.

Small synthetic molecule-stabilized RNA pseudoknot as an activator for -1 ribosomal frameshifting.

Matsumoto S, Caliskan N, Rodnina MV, Murata A, Nakatani K.
Nucleic Acids Res. 2018 Sep 19;46(16):8079-8089. doi: 10.1093/nar/gky689.

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Lavdovskaia E, Kolander E, Steube E, Mai MM, Urlaub H, Richter-Dennerlein R.
Nucleic Acids Res. 2018 Sep 19;46(16):8471-8482. doi: 10.1093/nar/gky701.

Crystal structure of the spliceosomal DEAH-box ATPase Prp2.

Schmitt A, Hamann F, Neumann P, Ficner R.
Acta Crystallogr D Struct Biol. 2018 Jul 1;74(Pt 7):643-654. doi: 10.1107/S2059798318006356. Epub 2018 Jun 8.

Velvet domain protein VosA represses the zinc cluster transcription factor SclB regulatory network for Aspergillus nidulans asexual development, oxidative stress response and secondary metabolism.

Thieme KG, Gerke J, Sasse C, Valerius O, Thieme S, Karimi R, Heinrich AK, Finkernagel F, Smith K, Bode HB, Freitag M, Ram AFJ, Braus GH.
J Cell Biol. 2018 Jul 2;217(7):2329-2340. doi: 10.1083/jcb.201712013. Epub 2018 May 10.

Xpo7 is a broad-spectrum exportin and a nuclear import receptor.

Aksu M, Pleiner T, Karaca S, Kappert C, Dehne HJ, Seibel K, Urlaub H, Bohnsack MT, Görlich D.
J Cell Biol. 2018 Jul 2;217(7):2329-2340. doi: 10.1083/jcb.201712013. Epub 2018 May 10.

Dynamics of ribosomes and release factors during translation termination in E. coli.

Adio S, Sharma H, Senyushkina T, Karki P, Maracci C, Wohlgemuth I, Holtkamp W, Peske F, Rodnina MV.
Elife. 2018 Jun 11;7. pii: e34252. doi: 10.7554/eLife.34252.

Three-dimensional single-molecule localization with nanometer accuracy using Metal-Induced Energy Transfer (MIET) imaging.

Karedla N, Chizhik AM, Stein SC, Ruhlandt D, Gregor I, Chizhik AI, Enderlein J.
J Chem Phys. 2018 May 28;148(20):204201. doi: 10.1063/1.5027074.

Validating Resolution Revolution.

Neumann P, Dickmanns A, Ficner R.
Structure. 2018 May 1;26(5):785-795.e4. doi: 10.1016/j.str.2018.03.004. Epub 2018 Mar 29.

Fluorescence lifetime correlation spectroscopy: Basics and applications.

Ghosh A, Karedla N, Thiele JC, Gregor I, Enderlein J.
Methods. 2018 May 1;140-141:32-39. doi: 10.1016/j.ymeth.2018.02.009. Epub 2018 Feb 16.

Structural basis for the regulatory interaction of the methylglyoxal synthase MgsA with the carbon flux regulator Crh in Bacillus subtilis.

Dickmanns A, Zschiedrich CP, Arens J, Parfentev I, Gundlach J, Hofele R, Neumann P, Urlaub H, Görke B, Ficner R, Stülke J.
J Biol Chem. 2018 Apr 20;293(16):5781-5792. doi: 10.1074/jbc.RA117.001289. Epub 2018 Mar 7.

Sensitivity-Enhanced Four-Dimensional Amide-Amide Correlation NMR Experiments for Sequential Assignment of Proline-Rich Disordered Proteins.

Wong LE, Maier J, Wienands J, Becker S, Griesinger C.
J Am Chem Soc. 2018 Mar 14;140(10):3518-3522. doi: 10.1021/jacs.8b00215. Epub 2018 Mar 6.

Sem1 links proteasome stability and specificity to multicellular development.

Kolog Gulko M, Heinrich G, Gross C, Popova B, Valerius O, Neumann P, Ficner R, Braus GH.
PLoS Genet. 2018 Feb 5;14(2):e1007141.doi: 10.1371/journal.pgen.1007141. eCollection 2018 Feb.

2017

Quantifying Microsecond Transition Times Using Fluorescence Lifetime Correlation Spectroscopy.

Ghosh A, Isbaner S, Veiga-Gutiérrez M, Gregor I, Enderlein J, Karedla N.
J Phys Chem Lett. 2017 Dec 21;8(24):6022-6028. doi: 10.1021/acs.jpclett.7b02707. Epub 2017 Dec 4

Three-Dimensional Reconstruction of Nuclear Envelope Architecture Using Dual-Color Metal-Induced Energy Transfer Imaging.

Chizhik AM, Ruhlandt D, Pfaff J, Karedla N, Chizhik AI, Gregor I, Kehlenbach RH, Enderlein J.
ACS Nano. 2017 Dec 26;11(12):11839-11846. doi: 10.1021/acsnano.7b04671.

Capturing the Asc1p/RACK1 microenvironment at the head region of the 40S ribosome with quantitative BioID.

Opitz N, Schmitt K, Hofer-Pretz V, Neumann B, Krebber H, Braus GH, Valerius O.
Mol Cell Proteomics. 2017 Dec;16(12):2199-2218. doi: 10.1074/mcp.M116.066654.

Slam protein dictates subcellular localization and translation of its own mRNA.

Yan S, Acharya S, Gröning S, Großhans J.
PLoS Biol. 2017 Dec 4;15(12):e2003315. doi: 10.1371/journal.pbio.2003315.

The RES complex is required for efficient transformation of the precatalytic B spliceosome into an activated Bact complex.

Bao P, Will CL, Urlaub H, Boon KL, Lührmann R.
Genes Dev. 2017 Dec 1;31(23-24):2416-2429. doi: 10.1101/gad.308163.117. Epub 2018 Jan 12

Mechanism of Transcription Anti-termination in Human Mitochondria.

Hillen HS, Parshin AV, Agaronyan K, Morozov YI, Graber JJ, Chernev A, Schwinghammer K, Urlaub H, Anikin M, Cramer P, Temiakov D.
Cell. 2017 Nov 16;171(5):1082-1093.e13. doi: 10.1016/j.cell.2017.09.035.

Structural Basis of Mitochondrial Transcription Initiation.

Hillen HS, Morozov YI, Sarfallah A, Temiakov D, Cramer P.
Cell. 2017 Nov 16;171(5):1072-1081.e10. doi: 10.1016/j.cell.2017.10.036.

Structures of transcription pre-initiation complex with TFIIH and Mediator.

Schilbach S, Hantsche M, Tegunov D, Dienemann C, Wigge C, Urlaub H, Cramer P.
Nature. 2017 Nov 9;551(7679):204-209. doi: 10.1038/nature24282.

INA complex liaises the F1Fo-ATP synthase membrane motor modules.

Naumenko N, Morgenstern M, Rucktäschel R, Warscheid B, Rehling P.
Nat Commun. 2017 Nov 1;8(1):1237. doi: 10.1038/s41467-017-01437-z.

Effect of Fusidic Acid on the Kinetics of Molecular Motions During EF-G-Induced Translocation on the Ribosome.

Belardinelli R, Rodnina MV.
Sci Rep. 2017 Sep 5;7(1):10536. doi: 10.1038/s41598-017-10916-8.

Tuning the ribosome: the influence of rRNA modification on eukaryotic ribosome biogenesis and function.

Sloan KE, Warda AS, Sharma S, Entian KD, Lafontaine DL, Bohnsack MT.
RNA Biol. 2017 Sep 2;14(9):1138-1152. doi: 10.1080/15476286.2016.1259781.

Cation selectivity of the presequence translocase channel Tim23 is crucial for efficient protein import.

Denkert N, Schendzielorz AB, Barbot M, Versemann L, Richter F, Rehling P, Meinecke M.
Elife. 2017 Aug 31;6. pii: e28324. doi: 10.7554/eLife.28324.

Data-driven coarse graining of large biomolecular structures.

Chen YL, Habeck M.
PLoS One. 2017 Aug 17;12(8):e0183057. doi: 10.1371/journal.pone.0183057.

Liquid-liquid phase separation of the microtubule-binding repeats of the Alzheimer-related protein Tau.

Ambadipudi S, Biernat J, Riedel D, Mandelkow E, Zweckstetter M.
Nat Commun. 2017 Aug 17;8(1):275. doi: 10.1038/s41467-017-00480-0.

Cryo-EM structure of a pre-catalytic human spliceosome primed for activation.

Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, Lührmann R, Stark H.
Cell. 2017 Aug 10;170(4):701-713.e11. doi: 10.1016/j.cell.2017.07.011.

Studying structure and function of spliceosomal helicases.

Ficner R, Dickmanns A, Neumann P.
Methods. 2017 Aug 1;125:63-69. doi: 10.1016/j.ymeth.2017.06.028.

Quick or Quality? How mRNA escapes nuclear quality control during stress.

Zander G, Krebber H.
RNA Biol. 2017 Jul 14:1-7. doi: 10.1080/15476286.2017.1345835.

Ribosome rearrangements at the onset of translational bypassing.

Agirrezabala X, Samatova E, Klimova M, Zamora M, Gil-Carton G, Rodnina MV, Valle M.
Sci Adv. 2017 Jun 7;3(6):e1700147. doi: 10.1126/sciadv.1700147.

Architecture of the RNA polymerase II-Paf1C-TFIIS transcription elongation complex.

Xu Y, Bernecky C, Lee CT, Maier KC, Schwalb B, Tegunov D, Plitzko JM, Urlaub H, Cramer P.
Nat Commun. 2017 Jun 6;8:15741. doi: 10.1038/ncomms15741.

Long-range allosteric regulation of the human 26S proteasome by 20S proteasome-targeting cancer drugs.

Haselbach D, Schrader J, Lambrecht F, Henneberg F, Chari A, Stark H.
Nat Commun. 2017 May 25;8:15578. doi: 10.1038/ncomms15578.

The highly conserved Asp23 family protein YqhY plays a role in lipid biosynthesis in Bacillus subtilis.

Tödter D, Gunka K, Stülke J.
Front Microbiol. 2017 May 19;8:883. doi: 10.3389/fmicb.2017.00883.

Conditional switch between frameshifting regimes upon translation of dnaX mRNA.

Caliskan N, Wohlgemuth I, Korniy N, Pearson M, Peske F, Rodnina MV.
Mol Cell. 2017 May 18;66(4):558-567.e4. doi: 10.1016/j.molcel.2017.04.023.

Core Mediator structure at 3.4 Å extends model of transcription initiation complex.

Nozawa K, Schneider TR, Cramer P.
Nature. 2017 May 11;545(7653):248-251. doi: 10.1038/nature22328.

The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction.

Fourmann JB, Dybkov O, Agafonov DE, Tauchert MJ, Urlaub H, Ficner R, Fabrizio P, Lührmann R.
Elife. 2016 Apr 26;5. pii: e15564. doi: 10.7554/eLife.15564.

Modeling of Biomolecular Assemblies with Cryo-EM Maps.

Habeck M.
Front Mol Biosci. 2017 Mar 22;4:15. doi: 10.3389/fmolb.2017.00015.

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.

Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Lührmann R.
Nature. 2017 Feb 16;542(7641):318-323. doi: 10.1038/nature21079.

A novel translational control mechanism involving RNA structures within coding sequences.

Jungfleisch J, Nedialkova D, Dotu I, Sloan KE, Martinez-Bosch N, Brüning L, Raineri E, Navarro P, Bohnsack MT, Leidel SA, Diez J.
Genome Res. 2017 Jan;27(1):95-106. doi: 10.1101/gr.209015.116.

Structural insights into the mechanism of the DEAH-box RNA helicase Prp43.

Tauchert MJ, Fourmann JB, Lührmann R, Ficner R.
Elife. 2017 Jan 16;6. pii: e21510. doi: 10.7554/eLife.21510.

Two distinct membrane potential-dependent steps drive mitochondrial matrix protein translocation.

Schendzielorz AB, Schulz C, Lytovchenko O, Clancy A, Guiard B, Ieva R, van der Laan M, Rehling P.
J Cell Biol. 2017 Jan 2;216(1):83-92. doi: 10.1083/jcb.201607066.

2016

mRNA quality control is bypassed for an immediate export of stress responsive transcripts.

Zander G, Hackmann A, Bender L, Becker D, Lingner T, Salinas G, Krebber H.
Nature. 2016 Dec 12. doi: 10.1038/nature20572.

Inferential Structure Determination of Chromosomes from Single-Cell Hi-C Data.

Carstens S, Nilges M, Habeck M.
PLoS Comput Biol. 2016 Dec 27;12(12):e1005292. doi: 10.1371/journal.pcbi.1005292.

Atg8 lipidation is coordinated in a PtdIns3P-dependent manner by the PROPPIN Atg21.

Krick R, Thumm M
Autophagy. 2016 Nov;12(11):2260-2261.

The oncogenic fusion proteins SET-Nup214 and sequestosome-1 (SQSTM1)-Nup214 form dynamic nuclear bodies and differentially affect nuclear protein- and poly(A)+ RNA export.

Port SA, Mendes A, Valkova C, Spillner C, Fahrenkrog B, Kaether C, Kehlenbach RH.
J Biol Chem. 2016 Oct 28;291(44):23068-23083. Epub 2016 Sep 9.

Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.

Richter-Dennerlein R, Oeljeklaus S, Lorenzi I, Ronsör C, Bareth B, Schendzielorz AB, Wang C, Warscheid B, Rehling P, Dennerlein S.
Cell. 2016 Oct 6;167(2):471-483.e10. doi: 10.1016/j.cell.2016.09.003.

Molecular architecture of the Saccharomyces cerevisiae activated spliceosome.

Rauhut R, Fabrizio P, Dybkov O, Hartmuth K, Pena V, Chari A, Kumar V, Lee CT, Urlaub H, Kastner B, Stark H, Lührmann R.
Science. 2016 Sep 23;353(6306):1399-1405. Epub 2016 Aug 25.

Localization of components of the RNA-degrading machine in Bacillus subtilis.

Cascante-Estepa N, Gunka K, Stülke J.
Front Microbiol. 2016 Sep 21;7:1492. eCollection 2016.

SCF ubiquitin ligase F-box protein Fbx15 controls nuclear co-repressor localization, stress response and virulence of the human pathogen Aspergillus fumigatus.

Jöhnk B, Bayram Ö, Abelmann A, Heinekamp T, Mattern D, Brakhage AA, Jacobsen ID, Valerius O, Braus GH.
PLoS Pathog. 2016 Sep 20;12(9):e1005899. doi: 10.1371/journal.ppat.1005899.

FQProfiler and RNP(xl): Open-Source Tools for Label-Free Quantification and Protein-RNA Cross-Linking Integrated into Proteome Discoverer.

Veit J, Sachsenberg T, Chernev A, Aicheler F, Urlaub H, Kohlbacher O.
J Proteome Res. 2016 Sep 2;15(9):3441-8. doi: 10.1021/acs.jproteome.6b00407.

A probabilistic model for detecting rigid domains in protein structures.

Nguyen T, Habeck M.
Bioinformatics. 2016 Sep 1;32(17):i710-i717. doi: 10.1093/bioinformatics/btw442.

Analysis of CRM1-dependent nuclear export in permeabilized cells.

Kehlenbach RH, Port SA.
Methods Mol Biol. 2016;1411:489-501. doi: 10.1007/978-1-4939-3530-7_30.

Analyzing Protein- Phosphoinositide Interactions with Liposome Flotation Assays.

Busse RA, Scacioc A, Schalk AM, Krick R, Thumm M, Kühnel K.
Methods Mol Biol. 2016;1376:155-62. doi: 10.1007/978-1-4939-3170-5_13.

Kinetics of spontaneous and EF-G-accelerated rotation of ribosomal subunits.

Sharma H, Adio S, Senyushkina T, Belardinelli R, Peske F, Rodnina MV.
Cell Rep. 2016 Aug 23;16(8):2187-2196. doi: 10.1016/j.celrep.2016.07.051.

The inhibition mechanism of human 20S proteasomes enables next-generation inhibitor design.

Schrader J, Henneberg F, Mata RA, Tittmann K, Schneider TR, Stark H, Bourenkov G, Chari A.
Science. 2016 Aug 5;353(6299):594-8. doi: 10.1126/science.aaf8993.

A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity.

Boesler C, Rigo N, Anokhina MM, Tauchert MJ, Agafonov DE, Kastner B, Urlaub H, Ficner R, Will CL, Lührmann R.
Nat Commun. 2016 Jul 5;7:11997. doi: 10.1038/ncomms11997.

Impact of the crystallization condition on Importin-beta conformation.

Tauchert M, Hemonnot C, Neumann P, Köster S, Ficner R, Dickmanns A.
Acta Crystallogr D Struct Biol. 2016 Jun;72(Pt 6):705-17. doi: 10.1107/S2059798316004940.

The adaptor protein CIN85 assembles intracellular signaling clusters for B cell activation.

Kühn J, Wong LE, Pirkuliyeva S, Schulz K, Schwiegk C, Fünfgeld KG, Keppler S, Batista FD, Urlaub H, Habeck M, Becker S, Griesinger C, Wienands J
Sci Signal. 2016 Jun 28;9(434):ra66. doi: 10.1126/scisignal.aad6275.

Structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A.

Aksu M, Trakhanov S, Görlich D.
Nat Commun. 2016 Jun 16;7:11952. doi: 10.1038/ncomms11952.

Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C.

Brown NG, VanderLinden R, Watson ER, Weissmann F, Ordureau A, Wu KP, Zhang W, Yu S, Mercredi PY, Harrison JS, Davidson IF, Qiao R, Lu Y, Dube P, Brunner MR, Grace CR, Miller DJ, Haselbach D*, Jarvis MA, Yamaguchi M, Yanishevski D, Petzold G, Sidhu SS, Kuhlman B, Kirschner MW, Harper JW, Peters JM, Stark H, Schulman BA.
Cell. 2016 Jun 2;165(6):1440-1453. doi: 10.1016/j.cell.2016.05.037.

Structural analysis of the spliceosomal RNA helicase Prp28 from the thermophilic eukaryote Chaetomium thermophilum.

Tauchert MJ, Ficner R.
Acta Crystallogr F Struct Biol Commun. 2016 May;72(Pt 5):409-16. doi: 10.1107/S2053230X16006038.

The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction.

Fourmann JB, Dybkov O, Agafonov DE, Tauchert MJ, Urlaub H, Ficner R, Fabrizio P, Lührmann R.
Elife. 2016 Apr 26;5. pii: e15564. doi: 10.7554/eLife.15564.

Choreography of molecular movements during ribosome progression along mRNA.

Belardinelli R, Sharma H, Caliskan N, da Cunha CEL, Peske F, Wintermeyer W, Rodnina MV.
Nat Struct Mol Biol. 2016 Apr;23(4):342-8. doi: 10.1038/nsmb.3193.

The DenA/ DEN1 interacting phosphatase DipA controls septa positioning and phosphorylation- dependent stability of cytoplasmatic DenA/DEN1 during fungal development.

Schinke J, Kolog Gulko M, Christmann M, Valerius O, Stumpf SK, Stirz M, Braus GH.
PLoS Genet. 2016 Mar 24;12(3):e1005949. doi: 10.1371/journal.pgen.1005949.

Molecular architecture of the human U4/U6.U5 tri-snRNP.

Heininger AU, Hackert P, Andreou AZ, Boon KL, Memet I, Prior M, Clancy A, Schmidt B, Urlaub H, Schleiff E, Sloan KE, Deckers M, Lührmann R, Enderlein J, Klostermeier D, Rehling P, Bohnsack MT.
Science. 2016 Mar 25;351(6280):1416-20. doi: 10.1126/science.aad2085.

Correction: Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation.

Pleiner T, Bates M, Trakhanov S, Lee CT, Schliep JE, Chug H, Böhning M, Stark H, Urlaub H, Görlich D.
Elife. 2016 Mar 16;5. pii: e15597. doi: 10.7554/eLife.15597.

Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways.

Agafonov D, Kastner B, Dybkov O, Hofele RV, Liu W, Urlaub H, Lührmann R, Stark H.
RNA Biol. 2016 Mar 3;13(3):320-30. doi: 10.1080/15476286.2016.1142038.

Changes of global expression and secondary metabolite accumulation during light-dependent Aspergillus nidulans development.

Bayram Ö, Feussner K, Dumkow M, Herrfurth C, Feussner I, Braus GH.
Fungal Genet Biol. 2016 Feb;87:30-53. doi: 10.1016/j.fgb.2016.01.004.

The devil is in the details – Comparison between COP9 signalosome (CSN) and the LID of the 26S proteasome.

Meister C, Kolog Gulko M, Köhler AM, Braus GH.
Curr Genet. 2016 Feb;62(1):129-36.

Retention and splicing complex (RES) - the importance of cooperativity.

Wysoczanski P, Zweckstetter M.
RNA Biol. 2016 Feb;13(2):128-33. doi: 10.1080/15476286.2015.1096484.

Nuclear Export of Pre-Ribosomal Subunits Requires Dbp5, but Not as an RNA-Helicase as for mRNA Export.

Neumann B, Wu H, Hackmann A, Krebber H.
PLoS One. 2016 Feb 12;11(2):e0149571. doi: 10.1371/journal.pone.0149571.

Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryote Chaetomium thermophilum.

Tauchert MJ, Fourmann JB, Christian H, Lührmann R, Ficner R.
Acta Crystallogr F Struct Biol Commun. 2016 Feb 1;72(Pt 2):112-20. doi: 10.1107/S2053230X15024498.

Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.

Pfaff J, Rivera Monroy, J, Jamieson, C, Rajanala, K, Vilardi, F, Schwappach B, Kehlenbach RH.
J Cell Sci. 2016 Feb 1;129(3):502-16. doi: 10.1242/jcs.179333.

2015

A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning.

Kirli K, Karaca S, Dehne HJ, Samwer M, Pan KT, Lenz C, Urlaub H, Görlich D.
Elife. 2015 Dec 17;4. pii: e11466. doi: 10.7554/eLife.11466.

Tuning and switching enantioselectivity of asymmetric carboligation in an enzyme through mutational analysis of a single hot spot.

Wechsler C, Meyer D, Loschonsky S, Funk LM, Neumann P, Ficner R, Brodhun F, Müller M, Tittmann K.
Chembiochem. 2015 Dec;16(18):2580-4. doi: 10.1002/cbic.201500529.

Directional transition from initiation to elongation in bacterial translation.

Goyal A, Belardinelli R, Maracci C, Milón P, Rodnina MV.
Nucleic Acids Res. 2015 Dec 15;43(22):10700-12. doi: 10.1093/nar/gkv869.

Dithiothreitol (DTT) Acts as a Specific, UV-inducible Cross-linker in Elucidation of Protein- RNA Interactions.

Zaman U, Richter FM, Hofele R, Kramer K, Sachsenberg T, Kohlbacher O, Lenz C, Urlaub H.
Mol Cell Proteomics. 2015 Dec;14(12):3196-210. doi: 10.1074/mcp.M115.052795.

Combining dehydration, construct optimization and improved data collection to solve the crystal structure of a CRM1-RanGTP-SPN1-Nup214 quaternary nuclear export complex.

Monecke T, Dickmanns A, Weiss MS, Port SA, Kehlenbach RH, Ficner R.
Acta Crystallogr F Struct Biol Commun. 2015 Dec 1;71(Pt 12):1481-7. doi: 10.1107/S2053230X15021524.

Structural Ensembles of Intrinsically Disordered Proteins Depend Strongly on Force Field: A Comparison to Experiment.

Rauscher S, Gapsys V, Gajda MJ, Zweckstetter M, de Groot BL, Grubmuller H.
J Chem Theory Comput. 2015 Nov 10;11(11):5513-24. doi: 10.1021/acs.jctc.5b00736.

Structural and Functional Characterization of CRM1-Nup214 Interactions Reveals Multiple FG-Binding Sites Involved in Nuclear Export.

Port SA, Monecke T, Dickmanns A, Spillner C, Hofele R, Urlaub H, Ficner R, Kehlenbach RH.
Cell Rep. 2015 Oct 27;13(4):690-702. doi: 10.1016/j.celrep.2015.09.042.

Simultaneous Measurement of the Three-Dimensional Orientation of Excitation and Emission Dipoles.

Karedla N, Stein SC, Hähnel D, Gregor I, Chizhik A, Enderlein J.
Phys Rev Lett. 2015 Oct 23;115(17):173002. doi: 10.1103/PhysRevLett.115.173002.

Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex.

Chug H, Trakhanov S, Hülsmann BB, Pleiner T, Görlich D.
Science. 2015 Oct 2;350(6256):106-10. doi: 10.1126/science.aac7420.

Hybrid structure of the type 1 pilus of uropathogenic Escherichia Coli.

Habenstein B, Loquet A, Hwang S, Giller K, Vasa SK, Becker S, Habeck M, Lange A.
Angew Chem Int Ed Engl. 2015 Sep 28;54(40):11691-5. doi: 10.1002/anie.201505065.

Structural Basis of Targeting the Exportin CRM1 in Cancer.

Dickmanns A, Monecke T, Ficner R.
Cells. 2015 Sep 21;4(3):538-68. doi: 10.3390/cells4030538. Review.

ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space.

Chari A, Haselbach D*, Kirves JM, Ohmer J, Paknia E, Fischer N, Ganichkin O, Moller V, Frye JJ, Petzold G, Jarvis M, Tietzel M, Grimm C, Peters JM, Schulman BA, Tittmann K, Markl J, Fischer U, Stark H.
Nat Methods. 2015 Sep;12(9):859-65. doi: 10.1038/nmeth.3493.

GraDeR: Membrane Protein Complex Preparation for Single-Particle Cryo-EM.

Hauer F, Gerle C, Fischer N, Oshima A, Shinzawa-Itoh K, Shimada S, Yokoyama K, Fujiyoshi Y, Stark H.
Structure. 2015 Sep 1;23(9):1769-75. doi: 10.1016/j.str.2015.06.029.

A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria.

Melin J, Kilisch M, Neumann P, Lytovchenko O, Gomkale R, Schendzielorz A, Schmidt B, Liepold T, Ficner R, Jahn O, Rehling P, Schulz C.
Biochim Biophys Acta. 2015 Aug;1853(8):1850-9. doi: 10.1016/j.bbamcr.2015.04.021.

Structures of intermediates during RES complex assembly.

Wysoczanski P, Becker S, Zweckstetter M.
Sci Rep. 2015 Jul 27;5:12545. doi: 10.1038/srep12545.

MD simulations and FRET reveal an environment-sensitive conformational plasticity of importin-β.

Halder K, Dölker N, Van Q, Gregor I, Dickmanns A, Baade I, Kehlenbach RH, Ficner R, Enderlein J, Grubmüller H, Neumann H.
Biophys J. 2015 Jul 21;109(2):277-86. doi: 10.1016/j.bpj.2015.06.014.

Integration of the catalytic subunit activates deneddylase activity in vivo as final step in fungal COP9 signalosome assembly.

Beckmann EA, Köhler AM, Meister C, Christmann M, Draht O, Rakebrandt N, Valerius O, Braus GH.
Mol Microbiol. 2015 Jul;97(1):110-24. doi: 10.1111/mmi.13017.

Fluctuations between multiple EF-G-induced chimeric tRNA states during translocation on the ribosome.

Adio S, Senyushkina T, Peske F, Fischer N, Wintermeyer W, Rodnina MV.
Nat Commun. 2015 Jun 15;6:7442. doi: 10.1038/ncomms8442.

Characterization of PROPPIN-Phosphoinositide Binding and Role of Loop 6CD in PROPPIN-Membrane Binding.

Busse RA, Scacioc A, Krick R, Pérez-Lara Á, Thumm M, Kühnel K.
Biophys J. 2015 May 5;108(9):2223-34. doi: 10.1016/j.bpj.2015.03.045.

Structure of the E. coli ribosome-EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM.

Fischer N, Neumann P, Konevega AL, Bock LV, Ficner R, Rodnina MV, Stark H.
Nature. 2015 Apr 23;520(7548):567-70. doi: 10.1038/nature14275.

HYCUD: a computational tool for prediction of effective rotational correlation time in flexible proteins.

Rezaei-Ghaleh N, Klama F, Munari F, Zweckstetter M.
Bioinformatics. 2015 Apr 15;31(8):1319-21. doi: 10.1093/bioinformatics/btu824.

PI3P binding by Atg21 organises Atg8 lipidation.

Juris L, Montino M, Rube P, Schlotterhose P, Thumm M, Krick R.
EMBO J. 2015 Apr 1;34(7):955-73. doi: 10.15252/embj.201488957.

Bayesian inference of initial models in cryo-electron microscopy using pseudo-atoms.

Joubert P, Habeck M.
Biophys J. 2015 Mar 10;108(5):1165-75. doi: 10.1016/j.bpj.2014.12.054.

Structural and biochemical analysis of the essential diadenylate cyclase CdaA from Listeria monocytogenes.

Rosenberg J, Dickmanns A, Neumann P, Gunka K, Arens J, Kaever V, Stülke J, Ficner R, Commichau FM.
J Biol Chem. 2015 Mar 6;290(10):6596-606. doi: 10.1074/jbc.M114.630418.

Ultra-stable and versatile widefield cryo-fluorescence microscope for single-molecule localization with sub-nanometer accuracy.

Li W, Stein SC, Gregor I, Enderlein J.
Opt Express. 2015 Feb 9;23(3):3770-83. doi: 10.1364/OE.23.003770.

Identification, characterization, and structure analysis of the cyclic di-AMP-binding PII-like signal transduction protein DarA.

Gundlach J, Dickmanns A, Schröder-Tittmann K, Neumann P, Kaesler J, Kampf J, Herzberg C, Hammer E, Schwede F, Kaever V, Tittmann K, Stülke J, Ficner R.
J Biol Chem. 2015 Jan 30;290(5):3069-80. doi: 10.1074/jbc.M114.619619.

The G-patch protein Spp2 couples the spliceosome-stimulated ATPase activity of the DEAH-box protein Prp2 to catalytic activation of the spliceosome.

Warkocki Z, Schneider C, Mozaffari-Jovin S, Schmitzová J, Höbartner C, Fabrizio P, Lührmann R.
Genes Dev. 2015 Jan 1;29(1):94-107. doi: 10.1101/gad.253070.114.

One Juliet and four Romeos: VeA and its methyltransferases.

82. Sarikaya-Bayram Ö, Palmer JM, Keller N, Braus GH, Bayram Ö.
Front Microbiol. 2015 Jan 20;6:1. doi: 10.3389/fmicb.2015.00001.

2014

Interaction of the intermembrane space domain of Tim23 protein with mitochondrial membranes.

Bajaj R, Munari F, Becker S, Zweckstetter M.
J Biol Chem. 2014 Dec 12;289(50):34620-6. doi: 10.1074/jbc.M114.595702.

Caught in the act: RNA-seq provides novel insights into mRNA degradation.

Gerwig J, Stülke J.
Mol Microbiol. 2014 Oct;94(1):5-8. doi: 10.1111/mmi.12769.

Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex.

Wysoczański P, Schneider C, Xiang S, Munari F, Trowitzsch S, Wahl MC, Lührmann R, Becker S, Zweckstetter M.
Nat Struct Mol Biol. 2014 Oct;21(10):911-8. doi: 10.1038/nsmb.2889.

Photo-cross-linking and high-resolution mass spectrometry for assignment of RNA-binding sites in RNA-binding proteins.

Kramer K, Sachsenberg T, Beckmann BM, Qamar S, Boon KL, Hentze MW, Kohlbacher O, Urlaub H.
Nat Methods. 2014 Oct;11(10):1064-70. doi: 10.1038/nmeth.3092.

Manipulation of fungal development as source of novel secondary metabolites for biotechnology.

Gerke J, Braus GH.
Appl Microbiol Biotechnol. 2014 Oct;98(20):8443-55. doi: 10.1007/s00253-014-5997-8.

Molecular basis of the dynamic structure of the TIM23 complex in the mitochondrial intermembrane space.

Bajaj R, Jaremko Ł, Jaremko M, Becker S, Zweckstetter M.
Structure. 2014 Oct 7;22(10):1501-11. doi: 10.1016/j.str.2014.07.015.

Telomerase RNA TLC1 shuttling to the cytoplasm requires mRNA export factors and is important for telomere maintenance.

Wu H, Becker D, Krebber H.
Cell Rep. 2014 Sep 25;8(6):1630-8. doi: 10.1016/j.celrep.2014.08.021.

Macromolecular assembly of the adaptor SLP-65 at intracellular vesicles in resting B cells.

Engelke M, Pirkuliyeva S, Kühn J, Wong L, Boyken J, Herrmann N, Becker S, Griesinger C, Wienands J.
Sci Signal. 2014 Aug 19;7(339):ra79. doi: 10.1126/scitranslmed.2005104.

The INA complex facilitates assembly of the peripheral stalk of the mitochondrial F1Fo-ATP synthase.

Lytovchenko O, Naumenko N, Oeljeklaus S, Schmidt B, von der Malsburg K, Deckers M, Warscheid B, van der Laan M, Rehling P.
EMBO J. 2014 Aug 1;33(15):1624-38. doi: 10.15252/embj.201488076.

A pre-ribosomal RNA interaction network involving snoRNAs and the Rok1 helicase.

Martin R, Hackert P, Ruprecht M, Simm S, Brüning L, Mirus O, Sloan KE, Kudla G, Schleiff E, Bohnsack MT
RNA. 2014 Aug;20(8):1173-82. doi: 10.1261/rna.044669.114.

Presequence recognition by the Tom40 channel contributes to precursor translocation into the mitochondrial matrix.

Melin J, Schulz C, Wrobel L, Bernhard O, Chacinska A, Jahn O, Schmidt B, Rehling P.
Mol Cell Biol. 2014 Sep 15;34(18):3473-85. doi: 10.1128/MCB.00433-14.

Control of the Diadenylate Cyclase CdaS in Bacillus subtilis: An Autoinhibitory Domain Limits c-di-AMP Production.

Mehne FM, Schroder-Tittmann K, Eijlander RT, Herzberg C, Hewitt L, Kaever V, Lewis RJ, Kuipers OP, Tittmann K, Stulke J.
J Biol Chem. 2014 Jul 25;289(30):21098-107. doi: 10.1074/jbc.M114.562066.

High-efficiency translational bypassing of non-coding nucleotides specified by mRNA structure and nascent peptide.

Samatova E, Konevega AL, Wills NM, Atkins JF, Rodnina MV.
Nat Commun. 2014 Jul 21;5:4459. doi: 10.1038/ncomms5459.

Remodelling of the active presequence translocase drives motor- dependent mitochondrial protein translocation.

Schulz C, Rehling P.
Nat Commun. 2014 Jul 10;5:4349. doi: 10.1038/ncomms5349.

Structural and functional analysis of the human spliceosomal DEAD-box helicase Prp28.

Möhlmann S, Mathew R, Neumann P, Schmitt A, Lührmann R, Ficner R.
Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1622-30. doi: 10.1107/S1399004714006439.

Programmed –1-frameshifting by kinetic partitioning during impeded translocation.

Caliskan N, Katunin VI, Belardinelli R, Peske F, Rodnina MV.
Cell. 2014 Jun 19;157(7):1619-31. doi: 10.1016/j.cell.2014.04.041.

Membrane-Bound Methyltransferase Complex VapA-VipC-VapB Guides Epigenetic Control of Fungal Development.

Sarikaya-Bayram O, Bayram O, Feussner K, Kim JH, Kim HS, Kaever A, Feussner I, Chae KS, Han DM, Han KH, Braus GH.
Dev Cell. 2014 May 27;29(4):406-20. doi: 10.1016/j.devcel.2014.03.020.

Allosteric control of the exportin CRM1 unraveled by crystal structure analysis.

Monecke T, Dickmanns A, Ficner R.
FEBS J. 2014 May 14. doi: 10.1111/febs.12842.

Quality control of spliced mRNAs requires the shuttling SR proteins Gbp2 and Hrb1.

Hackmann A, Wu H, Schneider UM, Meyer K, Jung K, Krebber H.
Nat Commun. 2014;5:3123. doi: 10.1038/ncomms4123.

GTP hydrolysis by EF-G synchronizes tRNA translocation on small and large ribosomal subunits.

Holtkamp W, Cunha C, Peske F, Konevega AL, Wintermeyer W, Rodnina MV.
EMBO J. 2014 May 2;33(9):1073-85. doi: 10.1002/embj.201387465.

Purification of protein complexes of defined subunit stoichiometry using a set of orthogonal, tag-cleaving proteases.

Frey S, Görlich D.
J Chromatogr A. 2014 Apr 11;1337:106-15. doi: 10.1016/j.chroma.2014.02.030.

A new set of highly efficient, tag-cleaving proteases for purifying recombinant proteins.

Frey S, Görlich D.
J Chromatogr A. 2014 Apr 11;1337:95-105. doi: 10.1016/j.chroma.2014.02.029.

Single-Molecule Metal Induced Energy Transfer (smMIET): Resolving nanometer distances at single molecule level.

Karedla N, Chizhik AI, Gregor I, Enderlein J.
Chemphyschem. 2014 Mar 17;15(4):705-11. doi: 10.1002/cphc.201300760.

Function and dynamics of slam in furrow formation in early Drosophila embryo.

Acharya S, Laupsien P, Wenzl C, Yan S, Großhans J.
Dev Biol. 2014 Feb 15;386(2):371-84. doi: 10.1016/j.ydbio.2013.12.022.

The YmdB phosphodiesterase is a global regulator of late adaptive responses in Bacillus subtilis.

Diethmaier C, Newman JA, Kovács AT, Kaever V, Herzberg C, Rodrigues C, Boonstra M, Kuipers OP, Lewis RJ, Stülke J.
J Bacteriol. 2014 Jan;196(2):265-75. doi: 10.1128/JB.00826-13.

SubtiWiki – a database for the model organism Bacillus subtilis that links pathway, interaction and expression information.

Michna RH, Commichau FM, Tödter D, Zschiedrich CP, Stülke J.
Nucleic Acids Res. 2014 Jan;42(Database issue):D692-8. doi: 10.1093/nar/gkt1002.

Insights into the activation of the helicase Prp43 by biochemical studies and structural mass spectrometry.

Christian H, Hofele RV, Urlaub H, Ficner R.
Nucleic Acids Res. 2014 Jan 1;42(2):1162-79. doi: 10.1093/nar/gkt985.

2013

The Velvet Family of Fungal Regulators Contains a DNA-Binding Domain Structurally Similar to NF-κB.

Ahmed, Y.L., Gerke, J., Park, H.-S., Bayram, Ö., Neumann, P., Min Ni, M., Dickmanns, A., Kim, S.C. , Yu, J.-H., Braus, G.H. and Ficner, R. .
PLoS Biol. 2013 Dec;11(12):e1001750. doi: 10.1371/journal.pbio.1001750.

Structural basis for cooperativity of CRM1 export complex formation.

Monecke T, Haselbach D, Voß B, Russek A, Neumann P, Thomson E, Hurt E, Zachariae U, Stark H, Grubmüller H, Dickmanns A, Ficner R.
Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):960-5. doi: 10.1073/pnas.1215214110.

Propranolol Restricts the Mobility of Single EGF-Receptors on the Cell Surface before Their Internalization.

Carolina Otero, Max Linke, Paula Sanchez, Alfonso González, Iwan A. T. Schaap
PLoS One. 2013 Dec 9;8(12):e83086. doi: 10.1371/journal.pone.0083086.

Cutting Edge: Feed-Forward Activation of Phospholipase Cγ2 via C2 Domain-Mediated Binding to SLP65.

Engelke M, Oellerich T, Dittmann K, Hsiao HH, Urlaub H, Serve H, Griesinger C, Wienands J.
J Immunol. 2013 Dec 1;191(11):5354-8. doi: 10.4049/jimmunol.1301326.

Monosome Formation during Translation Initiation Requires the Serine/Arginine-Rich Protein Npl3.

Baierlein C, Hackmann A, Gross T, Henker L, Hinz F, Krebber H.
Mol Cell Biol. 2013 Dec;33(24):4811-23. doi: 10.1128/MCB.00873-13.

A high-speed vertical optical trap for the mechanical testing of living cells at piconewton forces.

Bodensiek K, Li W, Sánchez P, Nawaz S, Schaap IA.
Rev Sci Instrum. 2013 Nov;84(11):113707. doi: 10.1063/1.4832036.

Energy barriers and driving forces in tRNA translocation through the ribosome.

Bock LV, Blau C, Schröder GF, Davydov II, Fischer N, Stark H, Rodnina MV, Vaiana AC, Grubmüller H.
Nat Struct Mol Biol. 2013 Nov 3. doi: 10.1038/nsmb.2690.

Phosphorylation Drives a Dynamic Switch in Serine/Arginine-Rich Proteins.

Xiang S, Gapsys V, Kim HY, Bessonov S, Hsiao HH, Möhlmann S, Klaukien V, Ficner R, Becker S, Urlaub H, Lührmann R, de Groot B, Zweckstetter M.
Structure. 2013 Oct 30. pii: S0969-2126(13)00365-1. doi: 10.1016/j.str.2013.09.014.

RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core.

Anokhina M, Bessonov S, Miao Z, Westhof E, Hartmuth K, Lührmann R.
EMBO J. 2013 Oct 30;32(21):2804-18. doi: 10.1038/emboj.2013.198.

Structural determinants and mechanism of mammalian CRM1 allostery.
Dölker, N., Blanchet, C.E., Voß, B., Haselbach, D., Kappel , C., Monecke,T., Svergun, D.I., Stark, H., Ficner, R., Zachariae, U., Grubmüller, H.and Dickmanns, A. .
Structure. 2013 Aug 6;21(8):1350-60. doi: 10.1016/j.str.2013.05.015.

Predicting the rotational tumbling of dynamic multidomain proteins and supramolecular complexes.

Rezaei-Ghaleh N, Klama F, Munari F, Zweckstetter M.
Angew Chem Int Ed Engl. 2013 Oct 18;52(43):11410-4. doi: 10.1002/anie.201305094.

Sub-ångström-resolution crystallography reveals physical distortions that enhance reactivity of a covalent enzymatic intermediate.

Lüdtke S, Neumann P, Erixon KM, Leeper F, Kluger R, Ficner R, Tittmann K.
Nat Chem. 2013 Sep;5(9):762-7. doi: 10.1038/nchem.1728.

Observation of a stable carbene at the active site of a thiamin enzyme.

Meyer D, Neumann P, Ficner R, Tittmann K
Nat Chem Biol. 2013 Aug;9(8):488-90. doi: 10.1038/nchembio.1275.

Inhibition of RNA helicase Brr2 by the C-terminal tail of the spliceosomal protein Prp8.

Mozaffari-Jovin S, Wandersleben T, Santos KF, Will CL, Lührmann R, Wahl MC.
Science. 2013 Jul 5;341(6141):80-4. doi: 10.1126/science.1237515.

Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system.

Ohrt T, Odenwälder P, Dannenberg J, Prior M, Warkocki Z, Schmitzová J, Karaduman R, Gregor I, Enderlein J, Fabrizio P, Lührmann R.
RNA. 2013 Jul;19(7):902-15. doi: 10.1261/rna.039024.113.

Essential genes in Bacillus subtilis: a re-evaluation after ten years.

Commichau FM, Pietack N, Stülke J.
Mol Biosyst. 2013 Jun 7;9(6):1068-75. doi: 10.1039/c3mb25595f.

Qualitative and quantitative characterization of protein-phosphoinositide interactions with liposome-based methods.

Busse RA, Scacioc A, Hernandez JM, Krick R, Stephan M, Janshoff A, Thumm M, Kühnel K.
Autophagy. 2013 May 1;9(5):770-7. doi: 10.4161/auto.23978.

conF and conJ contribute to conidia germination and stress response in the filamentous fungus Aspergillus nidulans.

Suzuki S, Sarikaya Bayram O, Bayram O, Braus GH.
Fungal Genet Biol. 2013 May 1. pii: S1087-1845(13)00078-9. doi: 10.1016/j.fgb.2013.04.008.

Dynamic localization of a transcription factor in Bacillus subtilis: The LicT antiterminator relocalizes in response to inducer availability.

Rothe FM, Wrede C, Lehnik-Habrink M, Görke B, Stülke J.
J Bacteriol. 2013 May;195(10):2146-54. doi: 10.1128/JB.00117-13.

Alternating sites reactivity is a common feature of thiamin diphosphate-dependent enzymes as evidenced by isothermal titration calorimetry studies of substrate binding.

Schröder-Tittmann K, Meyer D, Arens J, Wechsler C, Tietzel M, Golbik R, Tittmann k.
Biochemistry. 2013 Apr 16;52(15):2505-7. doi: 10.1021/bi301591e.

Structural plasticity in human heterochromatin protein 1β.

Munari F, Rezaei-Ghaleh N, Xiang S, Fischle W, Zweckstetter M.
PLoS One. 2013 Apr 9;8(4):e60887. doi: 10.1371/journal.pone.0060887.

Dual use of GTP hydrolysis by elongation factor G on the ribosome.

Carlos E. Cunha, Riccardo Belardinelli, Frank Peske, Wolf Holtkamp, Wolfgang Wintermeyer, Marina V. Rodnina
Translation 2013; 1:e24315; http://dx.doi.org/10.4161/trla.24315

Signal recognition initiates reorganization of the presequence translocase during protein import.

Lytovchenko O, Melin J, Schulz C, Kilisch M, Hutu DP, Rehling P.
EMBO J. 2013 Mar 20;32(6):886-98. doi: 10.1038/emboj.2013.23.

Control of Multicellular Development by the Physically Interacting Deneddylases DEN1/DenA and COP9 Signalosome.

Christmann M, Schmaler T, Gordon C, Huang X, Bayram O, Schinke J, Stumpf S, Dubiel W, Braus GH.
PLoS Genet. 2013 Feb;9(2):e1003275. doi: 10.1371/journal.pgen.1003275.

Dissection of the factor requirements for spliceosome disassembly and the elucidation of its dissociation products using a purified splicing system.

Fourmann JB, Schmitzová J, Christian H, Urlaub H, Ficner R, Boon KL, Fabrizio P, Lührmann R.
Genes Dev. 2013 Feb 15;27(4):413-28. doi: 10.1101/gad.207779.112.

Burial of the polymorphic residue 129 in amyloid fibrils of prion stop mutants.

Skora L, Fonseca-Ornelas L, Hofele RV, Riedel D, Giller K, Watzlawik J, Schulz-Schaeffer WJ, Urlaub H, Becker S, Zweckstetter M.
J Biol Chem. 2013 Feb 1;288(5):2994-3002. doi: 10.1074/jbc.M112.423715

2012

The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA.

Mozaffari-Jovin S, Santos KF, Hsiao HH, Will CL, Urlaub H, Wahl MC, Lührmann R.
Genes Dev. 2012 Nov 1;26(21):2422-34. doi: 10.1101/gad.200949.112.

DEAD-Box RNA helicases in Bacillus subtilis have multiple functions and act independently from each other.

Lehnik-Habrink M, Rempeters L, Kovács ÁT, Wrede C, Baierlein C, Krebber H, Kuipers OP, Stülke J.
J Bacteriol. 2013 Feb;195(3):534-44. doi: 10.1128/JB.01475-12.

Characterization of the velvet regulators in Aspergillus fumigatus.

Park HS, Bayram O, Braus GH, Kim SC, Yu JH.
Mol Microbiol. 2012 Nov;86(4):937-53. doi: 10.1111/mmi.12032.

Structural analysis of the C-terminal domain of the spliceosomal helicase Prp22.

Kudlinzki D, Schmitt A, Christian H, Ficner R.
Biol Chem. 2012 Sep 8;393(10):1131-40. doi: 10.1515/hsz-2012-0158.

Determinants of interaction specificity of the Bacillus subtilis GlcT antitermination protein: functionality and phosphorylation specificity depend on the arrangement of the regulatory domains.

Himmel S, Zschiedrich CP, Becker S, Hsiao HH, Wolff S, Diethmaier C, Urlaub H, Lee D, Griesinger C, Stülke J..
J Biol Chem. 2012 Aug 10;287(33):27731-42. doi: 10.1074/jbc.M112.388850.

MITRAC Links Mitochondrial Protein Translocation to Respiratory-Chain Assembly and Translational Regulation.

Mick DU, Dennerlein S, Wiese H, Reinhold R, Pacheu-Grau D, Lorenzi I, Sasarman F, Weraarpachai W, Shoubridge EA, Warscheid B, Rehling P.
Cell. 2012 Dec 21;151(7):1528-41. doi: 10.1016/j.cell.2012.11.053.

The channel-forming Sym1 protein is transported by the TIM23 complex in a presequence-independent manner.

Reinhold R, Krüger V, Meinecke M, Schulz C, Schmidt B, Grunau SD, Guiard B, Wiedemann N, van der Laan M, Wagner R, Rehling P, Dudek J.
Mol Cell Biol. 2012 Dec;32(24):5009-21. doi: 10.1128/MCB.00843-12

Structural basis for functional cooperation between tandem helicase cassettes in Brr2-mediated remodeling of the spliceosome.

Santos KF, Jovin SM, Weber G, Pena V, Lührmann R, Wahl MC.
Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17418-23. doi: 10.1073/pnas.1208098109.

It takes two to tango: PROPPINs use two phosphoinositide-binding sites.

Thumm M, Busse RA, Scacioc A, Stephan M, Janshoff A, Kühnel K, Krick R.
Autophagy. 2012 Oct 15;9(1). doi: 10.1073/pnas.1205128109

Methylation of lysine 9 in histone H3 directs alternative modes of highly dynamic interaction of heterochromatin protein hHP1β with the nucleosome.

Munari F, Soeroes S, Zenn HM, Schomburg A, Kost N, Schröder S, Klingberg R, Rezaei-Ghaleh N, Stützer A, Gelato KA, Walla PJ, Becker S, Schwarzer D, Zimmermann B, Fischle W, Zweckstetter M.
J Biol Chem. 2012 Sep 28;287(40):33756-65.

DExD/H-box RNA helicases in ribosome biogenesis.

Martin R, Straub AU, Doebele C, Bohnsack MT.
RNA Biol. 2013 Jan;10(1):4-18. doi: 10.4161/rna.21879

The Aspergillus nidulans MAPK Module AnSte11-Ste50-Ste7-Fus3 Controls Development and Secondary Metabolism

Özgür Bayram, Özlem Sarikaya Bayram, Yasar Luqman Ahmed, Jun-ichi Maruyama, Oliver Valerius, Silvio O. Rizzoli, Ralf Ficner, Stefan Irniger, Gerhard H. Braus
PLoS Genet. 2012 Jul;8(7):e1002816., doi:10.1371/journal.pgen.1002816

Structural and functional characterization of the two phosphoinositide binding sites of PROPPINs, a β-propeller protein family.

Roswitha Kricka, Ricarda A. Busseb, Andreea Scaciocb, Milena Stephanc, Andreas Janshoffc, Michael Thumm, Karin Kühnel
Proc Natl Acad Sci U S A. 2012 Jul 24;109(30):E2042-9. doi: 10.1073/pnas.1205128109

Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS.

Ohrt T, Prior M, Dannenberg J, Odenwälder P, Dybkov O, Rasche N, Schmitzová J, Gregor I, Fabrizio P, Enderlein J, Lührmann R.
RNA. 2012 Jun;18(6):1244-56. doi: 10.1261/rna.033316.112.

RNA degradation in Bacillus subtilis: an interplay of essential endo- and exoribonucleases.
Martin Lehnik-Habrink, Richard J. Lewis, Ulrike Mäder and Jörg Stülke

Mol Microbiol. 2012 84(6), 1005–1017, doi:10.1111/j.1365-2958.2012.08072.x

The COP9 signalosome counteracts the accumulation of cullin SCF ubiquitin E3 RING ligases during fungal development.

von Zeska Kress MR, Harting R, Bayram O, Christmann M, Irmer H, Valerius O, Schinke J, Goldman GH, Braus GH.
Mol Microbiol. 2012 Mar;83(6):1162-77. doi: 10.1111/j.1365-2958.2012.07999.x.

Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre.

Rasche N, Dybkov O, Schmitzová J, Akyildiz B, Fabrizio P, Lührmann R.
EMBO J. 2012 Mar 21;31(6):1591-604. doi: 10.1038/emboj.2011.502.

2011

Tim50's presequence receptor domain is essential for signal driven transport across the TIM23 complex.

Schulz C, Lytovchenko O, Melin J, Chacinska A, Guiard B, Neumann P, Ficner R, Jahn O, Schmidt B, Rehling P.
J Cell Biol. 2011 Nov 14;195(4):643-56. doi: 10.1083/jcb.201105098.

A Novel Factor Controlling Bistability in Bacillus subtilis: The YmdB Protein Affects Flagellin Expression and Biofilm Formation.

Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J.
J Bacteriol. 2011 Nov;193(21):5997-6007. doi: 10.1128/JB.05360-11.

The B-cell antigen receptor signals through a preformed transducer module of SLP65 and CIN85.

Thomas Oellerich, Vanessa Bremes, Konstantin Neumann, Hanibal Bohnenberger, Kai Dittmann, He-Hsuan Hsiao, Michael Engelke, Tim Schnyder, Facundo Batista, Henning Urlaub and Jürgen Wienands
The EMBO Journal, 5 August 2011; doi:10.1038/emboj.2011.251

RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y.

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J.
Mol Microbiol., 4 Aug 2011; doi: 10.1111/j.1365-2958.2011.07777.

RNase Y in Bacillus subtilis: a natively disordered protein that is the functional equivalent to RNase E from Escherichia coli.
Lehnik-Habrink M, Newman J, Rothe FM, Solovyova AS, Rodrigues C, Herzberg C, Commichau FM, Lewis RJ, Stülke J.
J Bacteriol, 2011 Oct;193(19):5431-41. doi: 10.1128/JB.05500-11.

Ran-dependent nuclear export mediators: a structural perspective.

Güttler T, Görlich D.
EMBO J. 2011 Aug 31;30(17):3457-74. doi: 10.1038/emboj.2011.287.

The mRNA export factor Npl3 mediates the nuclear export of large ribosomal subunits.

Hackmann A, Gross T, Baierlein C, Krebber H.
EMBO Rep. 2011 Sep 30;12(10):1024-31. doi: 10.1038/embor.2011.155.

The signaling tool box for tyrosine-based costimulation of lymphocytes.

Engels N, Wienands J.
Curr Opin Immunol. 2011 Jun;23(3):324-9. doi: 10.1016/j.coi.2011.01.005.

Spatiotemporal resolution of Ca2+ signaling events by real time imaging of single B cells.

Hanibal Bohnenberger, Thomas Oellerich, Michael Engelke, He-Hsuan Hsiao, Henning Urlaub, Jürgen Wienands
European journal of immunology, 25 May 2011; doi: 10.1002/eji.201041326

Prevention of cross-talk in conserved regulatory systems: identification of specificity determinants in RNA-binding anti-termination proteins of the BglG family.

Hübner S, Declerck N, Diethmaier C, Le Coq D, Aymerich S, Stülke J.
Nucleic Acids Res, May 2011; 39(10):4360-72. doi: 10.1093/nar/gkr021