+ SFB860 Integrative Structural Biology of Dynamic Macromolecular Assemblies
sfb860Goettingen UniversityMax-Plank-Institute

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Press releases> Press release



Prof. Dr. Ralf Ficner mail
Tel.: +49 (0)551-39-14072
Fax: +49 (0)551-39-14082

SFB860 Administration
Susanne van Beckum mail
Tel.: +49 (0)551-39-10972
Fax: +49 (0)551-39-14082

Abtl. Molekulare Strukturbiologie
Georg-August-Universität Göttingen
Justus-von-Liebig-Weg 11
37077 Göttingen

Introduction of SFB860

The dynamics of large macromolecular assemblies is a fundamental property that has great impact on their molecular and cellular function. This CRC will analyze the dynamics of several large macromolecular complexes with regard to their structures, their changes in conformation and composition, their interactions with other biological macromolecules, and with respect to their temporal and spatial location in the cell. Hence, the projects of the CRC focus on the determination of three-dimensional structures, the deduction of structure’s implication for the molecular and cellular function, the dynamic processes during assembly, remodelling and disassembly, the role of natively unfolded domains, the impact of posttranslational modifications on structure, function and dynamics, the conformational dynamics of functional complexes and their subunits, and kinetics and thermodynamics of macromolecular interactions. Studying the three-dimensional structure and the dynamics of single, average-size proteins is nowadays mostly a routine. However, due to the stunning complexity of large multi-protein- and ribonucleoprotein complexes, classical biochemical, biophysical and structure determination methods - when used exclusively - do not meet the requirements to comprehend such systems. Innovative strategies have to be developed for sample preparation, identification of macromolecular interaction networks, as well as for the analysis of compositional and structural dynamics. Hybrid approaches that combine single particle electron microscopy, crystallography, NMR spectroscopy, small angle X-ray scattering, single-molecule techniques and computational tools are required to generate testable atomic models of large macromolecular complexes. The CRC will bundle a wide spectrum of interdisciplinary competences present in Göttingen for the integrative analysis of a defined set of large macromolecular complexes, namely the spliceosome, the ribosome, the RNA degradosome, the mRNP locasome, the slam RNP, the mitochondrial translocase complex, nuclear export complexes, autophagy protein complexes, SLP-assembled signalosomes, the COP9 signalosome and the pyruvate dehydrogenase mulitenzyme complex. Since challenges and approaches are very similar for these functionally different macro¬molecular assemblies, the application of complementary biophysical and biochemical methods, as well as the exchange of experimental experiences and know-how will result in a significant added value for each project.

SFB 860 is funded by DFG (Deutsche Forschungsgemeinschaft)
1. funding period July 2010 - June 2014.
2. funding period July 2014 - June 2018.


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Publications> all Publications

  • 04.2016
    The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction.
  • 03.2016
    Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways.
  • 02.2016
    Retention and splicing complex (RES) - the importance of cooperativity.
  • 02.2016
    Nuclear Export of Pre-Ribosomal Subunits Requires Dbp5, but Not as an RNA-Helicase as for mRNA Export.
  • 02.2016
    Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryote Chaetomium thermophilum.

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